Localization of adenosine deaminase and adenosine deaminase complexing protein in rabbit heart. Implications for adenosine metabolism.

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Localization of adenosine deaminase and adenosine deaminase complexing protein in rabbit heart. Implications for adenosine metabolism.

The distribution of adenosine deaminase and adenosine deaminase complexing protein in rabbit heart has been compared using immunohistochemical staining procedures. Sections (4-5 microns) of tissue fixed in Clarke's solution or paraformaldehyde and embedded in paraffin were stained by the peroxidase anti-peroxidase method for adenosine deaminase or complexing protein, using affinity purified ant...

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Characterization of the adenosine deaminase-adenosine deaminase complexing protein binding reaction.

Glutaraldehyde-fixed membranes from rabbit kidney cortex were used to characterize binding of monomeric adenosine deaminase to the adenosine deaminase complexing protein. With the use of bovine adenosine deaminase it was shown that enzyme binding is a saturable, high affinity process. The K value for binding of the bovine enzyme was 11 nM. Maximum enzyme binding and rate of binding to a constan...

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Immunohistochemical Localization of Adenosine Deaminase

Aim of Investigation: Four peptides, substance P (SP), somatostatin (SS), cholesystokinin (CCK) and vasoactive intestinal polypeptide (VIP) have been found to occur, and to some extent coexist, in small sensory gangion peurones in the cat. These cells have cutaneous (and other) receptors responding to noxious mechanical, heat, cold, and combinations of these modalities, and to non-noxious stimu...

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Caffeine effect on adenosine deaminase catalysis: A new look at the effect of caffeine on adenosine deaminase activity

The effect of physiological concentrations of caffeine (purified from Persian tea) on adenosine deaminase (ADA) activity at physiological and pathological concentrations of adenosine (as substrate) in 50 mM Tris-HCl buffer (pH 7.3) at 37°C was investigated, using UV-VIS spectroscopy. ADA exhibited a bi-phasic activity behavior and both phases showed positive cooperativities indicating adenosine...

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Caffeine effect on adenosine deaminase catalysis: A new look at the effect of caffeine on adenosine deaminase activity

The effect of physiological concentrations of caffeine (purified from Persian tea) on adenosine deaminase (ADA) activity at physiological and pathological concentrations of adenosine (as substrate) in 50 mM Tris-HCl buffer (pH 7.3) at 37°C was investigated, using UV-VIS spectroscopy. ADA exhibited a bi-phasic activity behavior and both phases showed positive cooperativities indicating adenosine...

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ژورنال

عنوان ژورنال: Circulation Research

سال: 1990

ISSN: 0009-7330,1524-4571

DOI: 10.1161/01.res.66.3.754